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Articles

Featured Application: 3-MCPD and Glycidyl Esters on Rxi-17Sil MS

The analysis of 3-MCPD and glycidyl esters using AOCS Cd 29c-13 is time consuming and exposes the GC-MS to harsh derivatization reagents that can limit column lifetime and increase maintenance costs. Here, we optimized the method using split injection, resulting in a time savings of 8 minutes, less damage from derivatization reagents, and the flexibility to use either PTV or split/splitless inlets.

Featured Application: Analysis of Melamine and Related Compounds on Raptor HILIC-Si

The analysis of melamine and related compounds is critical in protecting consumers from adulterated food products. While these highly polar analytes are difficult to retain and accurately quantify on many columns, the Raptor HILIC-Si column and LC-MS/MS method shown here allow quick and complete separations, so melamine and related compounds can be accurately identified and quantified even at residual levels.

Featured Application: Bisphenols on Raptor Biphenyl

The analysis of bisphenolic compounds that are used as alternatives to bisphenol A (BPA) is essential for monitoring exposure to and evaluating the safety of these emerging industrial chemicals. The LC-MS/MS method presented here provides excellent chromatographic peak shape and separation for an extensive list of BPA analogues in a fast, 8-minute analysis.

Featured Application: Raptor ARC-18によるカンナビノイドのプロファイル

カンナビノイドのこのUVを用いたシンプルなイソクラティック分析条件は、16種類のカンナビノイドを完全に分離します。これにより正確な効能試験を可能となり、プロファイルデータを報告できます。すべての化合物は9分という短時間でベースライン分離でき、ハイスループットカンナビ試験を必要とするラボに適しています。

Featured Application: LC Analysis of 4-MEI on Raptor FluoroPhenyl Columns

Raptor FluoroPhenyl columns perform well in both HILIC and reversed phase modes, giving method developers the flexibility to select the mode that performs best for their specific compounds of interest. Here we use a Raptor Fluorophenyl column in HILIC mode for LC analysis of 4-methylimidazole (4-MEI). This approach provides more retention than reversed phase mode and can be applied to determining 4-MEI levels in caramel colorings to monitor formulations and also in foods and beverages to assess potential exposure.

Featured Application:LC-MS/MSによるRaptor C18 を用いたマルチクラス動物用医薬品分析

食用動物への抗生物質の使用は、薬剤耐性菌を増加させる可能性があるため、公衆衛生上および安全上の懸念事項です。動物組織や食品における最大残留濃度(MRL)の規制を満たすためには、様々な抗生物質に対して高感度かつ効率的で信頼性の高い分析法が必要です。Raptor C18は残留抗生物質分析に理想的です。

Featured Application: Multiresidue Pesticide Mix Extracted from Brown Rice Flour Using QuEChERS Extraction and Cleanup

Multiresidue pesticides analysis presents many challenges, from extensive lists of chemically diverse analytes to the negative effects of complex matrix components. The optimized chromatographic method described here allows the analysis of hundreds of compounds in as little as 7.5 minutes with a total cycle time under 10 minutes, ensuring fast, accurate sample screening and increased lab productivity.

Featured Application: Raptor BiphenylによるカンナビスCBDオイル中マイコトキシン分析

マイコトキシン分析における煩雑で時間のかかるイムノアフィニティカラムサンプルによる前処理は、簡単なパススルーSPEサンプル精製に置き換えることができます。 Raptor Biphenylカラムを使用したLC-MS / MS分析と組み合わせることで、2 ng / gのアフラトキシンとオクラトキシンの優れたクロマトグラフィー結果が得られ、カンナビス中マイコトキシンのハイスループット分析が可能です。

Featured Application: Mycotoxins in Peanut Powder on Raptor Biphenyl

The method developed here provides a fast analysis of mycotoxins in peanut powder. Baseline separation of 12 FDA and EU regulated mycotoxins was achieved in a 5.5 minute analysis (7 minute total cycle time). This method can be applied to different matrices using either matrix-matched calibration or a stable isotope dilution assay. Sample throughput can be greatly increased by the short cycle time for each run.

Featured Application: Mycotoxins on Raptor FluoroPhenyl

Mycotoxin contamination of crops is not only a public health concern, but it also has a huge impact on the global economy. This highly selective LC-MS/MS analysis of 20 mycotoxins in just 9 minutes allows scientists to accurately identify toxins, to monitor food products for contamination, and to support control strategies that prevent economic loss.

A Comprehensive Approach to Pesticide Residue Testing, Including Non-Target Analysis, for Fruits, Vegetables, and Nuts, Using QuEChERS, LC-MS/MS, and GCxGC-TOFMS

Food commodities that varied in water, fat, and pigment content were fortified with pesticides and processed using a QuEChERS sample preparation technique. Samples were analyzed by both GCxGC-TOFMS and LC-MS/MS, and good recoveries were obtained for most pesticides in most commodities.

Analysis of Nicotine and Impurities in Electronic Cigarette Solutions and Vapor

Despite the growing popularity of electronic cigarettes, relatively little work has been done to characterize their vapor. In this study, we developed a quick, simple thermal desorption device for sampling vapor, as well as methods for analyzing vapor and solutions to determine nicotine content (GC-FID) and impurity profiles (GC-MS). This novel approach, which utilizes an Rtx-VMS column, provides detectable levels of volatile organic compounds (VOCs) and semivolatile organic compounds (SVOCs) from a single 40 mL puff.

Analysis of Nitrofurans in Honey

Nitrofurans are a class of veterinary antibiotics used to increase growth rate and prevent or treat disease in animals. Determining levels of nitrofurans in animal tissue, or even products such as honey, is important in studying drug resistance and allergies in humans. The Ultra C18 HPLC column is an excellent choice for LC-MS/MS analysis of nitrofurans at low levels in complex matrices such as honey. Here we show excellent sensitivity, resolution, and peak shape at trace levels.

QuEChERS法とSPEによる前処理を用いた GC-TOFMS および GC-MS/MSによるマテ茶中の多環芳香族炭化水素(PAHs)の分析

迅速かつ正確なマテ茶中のPAH分析手法について検討しました。試料前処理には、QuEChERS法を改良し、固相抽出による精製をおこないました。GC-TOFおよびGC-MS/MSの両方を使用し、現実的な分析時間の維持と同重体の分離を最適化した分析をおこないました。

Can Nonmatching Mycotoxin SIDA Internal Standards Be Used for Stable Isotope Dilution Assay Calibration?

Stable isotope dilution assay (SIDA) is a calibration technique that can be used to offset matrix effects. However, care must be given to choosing appropriate internal standards. This article demonstrates the importance of using a labeled internal standard that matches the target analyte for mycotoxin SIDA analysis.

Comparing Pesticide Residues in Amish and Commercially Grown Strawberries and Spinach Using QuEChERS, Various dSPE Sorbents, and GC-TOFMS

QuEChERS extraction, dSPE cleanup, and GC-TOFMS analysis were used to assess pesticide residue levels in strawberry and spinach from both commercial and Amish growers. Various dSPE sorbent products were compared to determine which were most effective.

Comprehensive Pesticide Residue Analysis by LC-MS/MS Using an Ultra Aqueous C18 Column

Analysis of pesticide residues in foods is complicated by the large number of target compounds. LC-MS/MS analysis using an Aqueous C18 column provides more comprehensive results than GC alone.

Determining Pesticides in Dietary Supplements with QuEChERS Extraction, Cartridge SPE, and GCxGC-TOFMS

The novel approach used here combines QuEChERS extraction, cartridge SPE cleanup, and GCxGC-TOFMS analysis, resulting in good recoveries for a wide range of pesticides in dietary supplements. Matrices include dandelion root, sage, and a multi-herb finished product.
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Developing New Methods for Pesticides in Dietary Supplements

QuEChERS is a simple, effective approach to sample prep that can be applied to the analysis of pesticides in dietary supplements. Here, we demonstrate a QuEChERS, cSPE, GC-TOFMS procedure that results in good recoveries for a wide range of pesticides in dandelion root. (PDF - 0kB)

Evaluation of Dispersive and Cartridge Solid Phase Extraction (SPE) Cleanups for Multiresidue Pesticides in QuEChERS Extracts of Finished Tobacco Using GCxGC-TOFMS

We compared the efficacy of dSPE and cSPE cleanup methods for multiresidue pesticide analysis of QuEChERS extracts of finished tobacco products using GCxGC-TOFMS. Cleanup with dSPE tubes containing 7.5 mg of GCB and 25 mg of PSA produced better results than other methods, with average recoveries of 92% and 91% for 500 ppb and 50 ppb fortifications respectively.
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Fast, Simple QuEChERS Extraction and Cleanup of Pesticide Residue Samples

QuEChERS dSPE methods simplify extract cleanup, reduce material costs, and improve sample throughput. Here we demonstrate the effectiveness of QuEChERS sample cleanup using a multiresidue analysis of pesticides on strawberries. (PDF - 0kB)

Fast, Simple Sample Cleanup

Quick, Easy, Cheap, Effective, Rugged, and Safe, the QuEChERS ("catchers") method for extracting pesticides from food is based on USDA research and employs a novel dispersive solid phase extraction cleanup (dSPE). QuEChERS methods are convenient, rugged methods that simplify extract cleanup, reduce material costs, and improve sample throughput. Here we demonstrate the effectiveness of QuEChERS sample cleanup using a multiresidue analysis of pesticides on strawberries.

GC/MS Analysis of Melamine and Cyanuric Acid Below
1 μg/g in Infant Formula

The establishment of a 1 µg/g safety threshold for melamine in infant foods has led to a need for sensitive methods. Here we established GC/MS conditions for highly reproducible analyses and evaluated the effectiveness of both solvent-based and matrix-matched standards. Using this method, melamine and cyanuric acid were reliably detected at and below 1 µg/g in infant formula.

High Sensitivity Melamine GC/MS Analysis of Cat Food

Melamine contamination was implicated in a large pet food recall that occurred in 2007 when animals died after eating contaminated pet food. Here, a modified GC/MS method, based on an FDA method, was used to analyze for melamine & related compounds cyanuric acid, ammelide, and ammeline in dry cat food. Analytes were easily identified by retention time matching and mass spectra.

High-Quality Analysis of Pesticides in Cannabis Using QuEChERS, Cartridge SPE Cleanup, and GCxGC-TOFMS

As medical marijuana is more frequently prescribed, patient safety must be ensured. Pesticide residue testing is an important part of assuring safe product is dispensed, but analysis can be extremely challenging due to matrix complexity. The use of QuEChERS, cartridge SPE cleanup, and GCxGC-TOFMS as presented here, produces high-quality quantitative data for this difficult analysis.

脂肪酸メチルエステル (FAMEs)の高分離GC分析

キャピラリーGCは、食品中の総脂質含有量、トランス脂肪酸含有量および総オメガ-3多価不飽和脂肪酸含有量の測定にとても便利です。キャピラリーカラムの選択は、どのような情報を必要とするかによって異なります。ここで紹介しているように、必要とする脂肪酸分析がどのようなものであれ、Restekは正確な測定のサポートする安定した性能の分析カラムと混合標準物質を提供いたします。

Highly Reproducible Detailed cis/trans FAMEs Analysis Ensured by New Optimized Rt-2560 Column Manufacturing and Application-Specific QC Test

Even minor variations in manufacturing processes can potentially have detrimental effects on column-to-column reproducibility. As shown here, Rt-2560 GC columns produced using a newly updated and optimized manufacturing process exhibit high reproducibility and provide consistent performance for detailed cis/trans FAMEs analysis.
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HPLC Analysis of Glucosinolates in Vegetable Extracts without Ion Pairing Using an Ultra Aqueous C18 Column

Glucosinolates with hydrophilic functional groups are very difficult to retain by conventional reversed phase HPLC, and when the functional groups in a sample are diverse, polarity can differ widely, complicating the problem. Until now, reversed phase HPLC with an ion pairing reagent has been the inconvenient, but necessary, approach to these analyses. This 2-page note describes a column and conditions for analyses of glucosinolates, without ion pairing. (PDF - 0kB)

Increase Data Quality for Sulfonamide Residue Analysis by HPLC and UHPLC Using Unique Biphenyl Column Selectivity

The unique selectivity and high retention of Biphenyl columns produce complete separation of sulfonamide residues and benefit both UV and MS detection. In addition, Biphenyl columns in a UHPLC format allow faster sample throughput when testing for these antibiotics.

Malachite Green and Leucomalachite Green Analysis

Illegal use of malachite green (MG), an inexpensive fungicide, can allow MG to enter water cycles, where it is easily absorbed by fish tissue, and thus enters the human food supply. Reversed phase HPLC often is used to analyze for MG and its stored metabolite, leucomalachite green. Methods that facilitate detection of both compounds are discussed.

Mitigating Matrix Effects: Examination of Dilution, QuEChERS, and Calibration Strategies for LC-MS/MS Analysis of Pesticide Residues in Diverse Food Types

LC-MS/MS is popular for monitoring pesticide residues in food due to its selectivity and sensitivity; however, matrix effects can cause poor data quality and difficult quantification. We evaluated the relative effectiveness of sample dilution, QuEChERS cleanup, solvent-based calibration, and matrix-matched calibration strategies to mitigate matrix effects in celery, kale, avocado, lime, and brown rice flour.

Monitor Anitoxidants in Tea Extract

An Ultra Aqueous C18 column enables you to use the conditions most effective for this analysis: gradient elution in high aqueous mobile phases. The C18 chains will not collapse in the presence of a high water content. Use this chromatographic system to extract data for specific compounds of interest and to manually inspect spectra for phenolic glycosides, esters of phenolic acid, or other compounds.
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New Rxi-PAH GC Column; Resolve Important Isobaric Polycyclic Aromatic Hydrocarbons for Food Safety and Environmental Methods

Separate isobaric polycyclic aromatic hydrocarbons, including priority EFSA PAH4 compounds benz[a]anthracene, chrysene, benzo[b]fluoranthene, and benzo[a]pyrene, easily and accurately on an Rxi-PAH column. Whether you need more resolution or faster analysis, these new GC columns offer the selectivity and efficiency you need for food safety and environmental PAH analysis. (PDF - 0kB)

Prepare Olive Oil Samples for Pesticide Residue Analysis in Half the Time With a Fraction of the Solvent Using dSPE

Simplify and speed up sample preparation with Resprep dSPE tubes! Here we show the extraction and clean-up of pesticide residues from olive oil samples—twice as fast as GPC, with only a fraction of the solvent required for conventional SPE.

Quick Reference Guide to GC Consumables by Instrument

Use our chart to quickly find the GC inlet liners, syringes, and septa that are recommended specifically for your instrument. Follow the links to our product pages for fast, online purchasing.

Rapid Characterization of Garlic Volatiles—No Sample Prep Required!

Chromatographic methods for garlic and garlic powder are used by the food and dietary supplement industries to monitor product quality. Here we present a headspace gas chromatography mass spectrometry (HS GC/MS) method for garlic flavor and odor components using an Rxi-5ms column. This method eliminates sample preparation making the bench work simple and fast. The experimental set-up is ideal for both screening analysis and low-level trace analysis.

Rapid Screening Method for Carbamates in Orange Oil

EPA Method 531.1 addresses the analysis of carbamate pesticides in water, but not in more complex matrices, which often contain interferences and require time-consuming sample preparation. We developed an easy, accurate screening method for carbamates in a complex matrix using an Ultra Carbamate HPLC column in conjunction with the Leco Unique TOF- MS. This method requires no sample preparation and provides fast analysis times, significantly increasing sample throughput.

Rapid, Reproducible HPLC Analysis for Flavonoids in Cocoa

We separated flavonoids in cacao or cocoa powder samples and returned conditions to the initial mobile phase composition in 15 minutes. For compounds common to both samples, retention times were equal to within 0.01 seconds. Flavonoid marker compound profiles for 38%, 49%, and 65% cacao were easily distinguished.

Robust 9-Minute GC Analysis of Cholesterol

Conditions are established for analyzing both derivatized and underivatized cholesterol on a highly inert Rxi-5ms column. Methods described include both an isothermal analysis for use when interferences are minimal, but sample throughput is critical, and a temperature program for use when separation of analytes from contaminants or interfering compounds is the primary concern. Results for both derivatized and underivatized samples were highly reproducible.

Rt-2560による牛乳中脂肪酸の頑健な分析:
ユーザーによるカラムの耐久性評価

乳脂肪中の脂肪酸の分析は重要なテストです。Eurofins Analytik GmbHはこの難しいマトリクスを使用してRt-2560カラムの安定性を評価しました。 225回の注入後も一貫した保持時間と分離が達成され、カラムの頑健性が実証されました。

Rt-2560 Columns Ensure Accurate, Reliable AOAC 996.06 and AOCS Ce 1j-07 FAMEs Analysis

Restek has optimized the manufacturing process and implemented a new, application-specific QC testing procedure for all new Rt-2560 columns (cat.# 13198). Here we demonstrate that, Rt-2560 columns produced using the optimized process exhibit excellent performance for both AOAC 996.06 and AOCS Ce 1j-07 FAMEs analysis and meet all method requirements.

Simple, Reliable HPLC Analyses of Organic Acids

Commonly used organic acid methods (e.g. AOAC method 986.13) depend on reversed phase HPLC and C18 columns, however these columns are vulnerable to phase collapse when used with the aqueous mobile phases necessary for optimal organic acid analysis. Restek’s Ultra Aqueous C18, Allure Aqueous C18, and Allure Organic Acids columns all withstand phase collapse and resolve organic acids in a 100% aqueous mobile phase, compared to a conventional C18 column which shows a complete loss of retention.
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Stabilwax-MS Columns—Thermally Stable, High Polarity GC Columns for Flavor, Food, Fragrance, Industrial Chemical, and Solvent Analyses

Low-bleed Stabilwax-MS columns produce excellent results for the analysis of trace levels of polar compounds by GC-MS. These columns are ideal for a wide range of polar analytes including FAMEs, flavor compounds, essential oils, solvents, aromatics, acrolein/acrylonitrile, and oxygenated compounds. (PDF - 0kB)
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Thermal Desorption: A Practical Applications Guide: IV. Food, Flavour, Fragrance & Odour Profiling

Thermal desorption is used extensively for measuring volatile and semivolatile components in foods, flavors, fragrances, and odors. This 28-page publication from Markes International Ltd. presents several key applications. (PDF - 0kB)

Using Thermal Desorption to Enhance Aroma Profiling by GC/MS

Thermal desorption offers an automatic, high-sensitivity alternative to conventional liquid extraction methods for aroma profiling by GC/MS. It allows vapor profile constituents to be cleanly separated from the sample matrix and often facilitates selective purging of volatile interferences. This ensures that the vapor profile analyzed is most representative of the aroma perceived by consumers and that key compounds can be identified and measured at the lowest levels possible.

USLC Columns Put the Right Tools in Your LC Method Development Toolbox

Column selectivity has the most significant influence on chromatographic peak separation, or resolution, so choosing the right column can greatly speed up HPLC and UHPLC method development. In this article, we discuss column choice and identify a set of just 4 stationary phases—Restek’s USLC column set—that encompasses the widest range of reversed phase selectivity available today.