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- Separates a wider range of compounds than other phenyl and C18 chemistries.
- Increases sensitivity and selectivity in LC-MS while using simple, MS-friendly mobile phases.
- Restek’s most popular LC phase—available on traditional FPP and SPP core-shell silica.
Launched in 2005, the innovative Biphenyl (USP L11) is Restek’s most popular LC stationary phase because it is particularly adept at separating compounds that are hard to resolve or that elute early on C18 and other phenyl chemistries. As a result, rugged Biphenyl columns are extremely useful for fast separations—even with isobars—in drug, metabolite, and other analyses, especially those that require a mass spectrometer (MS). Increasing retention of early-eluting compounds can limit ionization suppression, and the Restek Biphenyl's heightened selectivity helps eliminate the need for complex mobile phases that are not well-suited for MS detection.
With ten years of unrivaled performance, Restek's time-tested Biphenyl has been proven to be the ideal column for clinical diagnostic, pain, pharma, and environmental labs seeking universal utility for mass spec applications. Restek was the first to bring you the benefits of the Biphenyl ligand, and our LC group has the veteran technical expertise to supply and support your lab better than anyone else.
The Restek Biphenyl is the Right LC Column in Any Format:
FEATURED
Raptor SPP Columns: A new species of column that combines the speed of superficially porous particles (SPP or “core-shell”) with time-tested Restek Biphenyl selectivity.
Force FPP Columns: Long-lasting, reproducible, and fully scalable FPP (fully porous particle) columns that let you easily transfer methods across your entire lab, from trusted-but-aged HPLCs to cutting-edge UHPLCs.
ALSO AVAILABLE
Ultra Columns: High-purity, type-B silica that minimizes activity and creates high-density bonding for reliable HPLC use.
Viva Columns: Rugged, spherical particles with a wide 300 Å pore size and narrow distribution that are designed for large-molecule separations.
DECONSTRUCTING THE BIPHENYL
With USLC technology, Restek's LC experts have defined our columns' selectivity based on molecular interactions. The more rings shown, the more significant a role played in solute retention. Learn more at www.restek.com/uslc
Properties:
- Increased retention for dipolar, unsaturated, or conjugated solutes.
- Enhanced selectivity when used with methanolic mobile phase.
- Ideal for increasing sensitivity and selectivity in LC-MS analyses.
Switch to Biphenyl when:
- Limited selectivity is observed on a C18.
- You need to increase retention of hydrophilic aromatics.
Raptor Biphenyl LC カラム (USP L11)
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Force Biphenyl LC カラム (USP L11)
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Ultra Biphenyl カラム (USP L11)粒子径：3 μmまたは5 μm、球状 細孔径：100Å 炭素含有率：15% エンドキャップ： あり pH範囲：2.5～8.0 温度上限： 80℃ USPコード：L11 固定相カテゴリー：Phenyl リガンドタイプ：Biphenyl |
Ultra Biphenyl Prep カラムOur broadest selection of stationary phases, including unique phases. High-density bondings for maximum retention. High-purity, Type-B silica gives excellent peak shapes for a wide range of compounds. Note: We strongly recommend ordering a semi-prep or prep column only after evaluating the desired separation on an equivalent analytical-scale column. Because we cannot reuse a column or the silica it contains once it has left our facility, we cannot accept returns of large-scale columns. |
Viva Biphenyl カラム (USP L11)Stationary Phase Category: phenyl (L11) Ligand Type: Biphenyl Particle: 5 µm, spherical Pore Size: 300 Å Carbon Load: 7% End-Cap: yes Surface Area: 100 m2/g pH Range: 2.5 to 8 Maximum Temperature: 80 °C |
EXP ダイレクト コネクト ホルダー
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Raptor EXP ガードカラムカートリッジ
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Force EXPガードカラムカートリッジ
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HPLC & UHPLC用EXP フィッティング 10-32フィッティング、 1/16インチ配管用EXPハンドタイトフィッティング
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Ultra ガードカートリッジParticle size: 5 µm spherical For a detailed description of the stationary phase in this guard cartridge, see the product page for an analytical column with the same silica and stationary phase (e.g., Ultra C18). |
Viva ガードカートリッジParticle size: 5 µm spherical For a detailed description of the stationary phase in this guard cartridge, see the product page for an analytical column with the same silica and stationary phase (e.g., Viva C18). |
UltraShield UHPLC プレカラム フィルター
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Overcoming the Effects of Highly Organic Protein Precipitation Extracts on LC Peak Shape Using Direct InjectionProtein precipitation is frequently used to minimize matrix impact when analyzing biological samples. However, the effects of highly organic protein precipitation sample extracts on LC peak shape can negatively impact accurate quantification. Dilution or further sample preparation steps are often used to minimize these effects; however, here we show that direct injection of sample extracts is a viable option that can be used to prevent peak distortion, while avoiding the time and variability associated with additional sample preparation. |
LC-MS/MSを用いた全血中免疫抑制剤のハイスループット分析臓器移植の成功は、免疫抑制剤の正確かつタイムリーな分析に大きく依存します。本検討では、全血中のシクロスポリンA、タクロリムス、シロリムスおよびエベロリムスの迅速かつ正確な方法を開発しました。ここでは、Raptor Biphenylカラムを用いたLC-MS/MS分析とタンパク質沈殿による前処理のみを組合わせ、マトリックスの影響を受けずに、3分という高速分析をおこなうことができました。直線性、堅牢性、精確さおよび精度において優れた結果が得られ、この方法が治療薬物モニタリングにおけるハイスループット分析に適していることが示されました。 |
The Role of Selectivity in Liquid Chromatography Method DevelopmentDr. Schug, Associate Professor and Shimadzu Distinguished Professor of Analytical Chemistry in the Department of Chemistry and Biochemistry at The University of Texas at Arlington, discusses the role of column selectivity in liquid chromatography method development. |
Raptor Biphenyl LCカラムを用いた3種類の甲状腺ホルモンの高速かつ高感度なLC-MS/MS分析Raptor Biphenylカラムは甲状腺ホルモンの迅速かつ高感度な分析に最適です。ここで紹介するメソッドでは、2 pg/mL (T3)または5 pg/mL (T4 and rT3)といった濃度の甲状腺ホルモンを3.5分以内に測定可能です。この分析方法は低濃度の遊離甲状腺ホルモンの臨床分析に応用できます。 |
Raptor Biphenyl LC Columns BrochureRaptor LC columns combine the speed of superficially porous particles (i.e., SPP or “core-shell”) with the resolution of highly selective USLC technology. Featuring Restek's most popular LC stationary phase, the rugged Raptor Biphenyl is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). (PDF - 6MB) |
Raptor Biphenyl LC Columns Sales SheetFast, Rugged Raptor Columns with Time-Tested Selectivity: The industry-leading Biphenyl, first introduced by Restek in 2005, is our most popular LC stationary phase because it is particularly adept at separating compounds that are hard to resolve or that elute early on C18 and other phenyl chemistries. As a result, the rugged Raptor Biphenyl column is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). (PDF - 4MB) |
LCクロマトグラム
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 Bisphenols on Raptor Biphenyl 1.8 μmLC_FS0518 |
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